Abstract: Glucose, as the template compound, was modified by glycidyl methacrylate and copolymerized with acrylic acid and acrylamide. After freeze drying, the pH-sensitive glulose-based hydrogels were obtained. The experimental conditions were studied and the optimal conditions were the 1:4:4 mass ratio of glucose/acrylic acid/acrylic amide, the 0.5% dosage of cross-linking agent, initiator and reaction temperature at 65℃. And the swelling ratio of hydrogel obtained under these conditions was about 90.5 g/g. Additionally, it was found that the swelling ratio of the glulose-based hydrogel increased with time extension and finally approached equilibrium in certain pH medium. And the swelling ratio of the hydrogels in the intestinal fluid without enzymes (pH=7.4) was significantly higher than that in the gastric fluid without enzymes (pH=1.2). Moreover, the hydrogels could keep basically no degradation in the two kinds of medium. This showed certain application prospect in tissue engineering materials.