Abstract: To explore the better raw material for cellulose acetate film, cellulose isolated from Dendrocalamus sinicus (BC), bamboo pulp cellulose(PC) and commercially available microcrystalline cellulose(MC) were used as raw materials,which respectively react with acetic anhydride used as acetylating agent in presence of concentrated sulfuric acid used as catalyst, to prepare bamboo cellulose acetate from D. sinicus (BCA), bamboo pulp acetylated cellulose(PCA) and microcrystalline cellulose acetate(MCA). Three cellulose acetate films were then prepared and their properties were investigated. The results showed that the active hydroxyl of BC had the most substituted activity by acetyl groups. The content of acetyl group of BCA could reach 48.54%, slightly higher than that of MCA(46.06%) and PCA(44.13%). The BCA film's tensile strength and elongation at break of BCA film reached 45.97 MPa and 2.1%, which were significantly stronger than those of PCA film and MCA film. The transmittance of BCA film >90%. It also exhibited the strongest hydrophobicity, with the contact angle of 92.9°. Moreover, the surface of the BCA film was relatively smooth and the structure was the most compact. Additionally, BCA film also showed high thermal stability. Therefore, BC was the best raw material for the preparation of cellulose acetate film, while compared to PC and MC.